Report on the Verification of the Performance of a Method for the Detection of “Bt 63” Rice Using Real-Time PCR

In October 2006 the Joint Research Centre of the European Commission, namely the Community Reference Laboratory for GM Food and Feed (CRL-GMFF), received samples possibly containing Bt rice as tested by GeneScan with a “Bt 63” construct-specific method. The CRL-GMFF, as a result of the analyses conducted, suggested to the European Network of GMO Laboratories (ENGL) and to the EU enforcement laboratories the use of the detection method developed by Mäde et al. targeting the junction region between the cry1A(b)/cry1A(c) fusion protein and the nos terminator.